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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
26/02/2024 |
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Actualizado : |
26/02/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
COSTA, M.; MANSILLA, F.; MANUEL SALA, J.; SARAVIA, A.; UBIOS, D.; LORES, P.; CAPOZZO, A.V.; FREIRE, T. |
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Afiliación : |
MONIQUE COSTA, Laboratorio de Inmunomodulación y Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay; FLORENCIA MANSILLA, Instituto de Virología e Innovaciones Tecnológicas, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), INTA, Buenos Aires, Hurlingham, Argentina; JUAN MANUEL SALA, Estación Experimental Agropecuaria- Instituto Nacional de Tecnología Agropecuaria (INTA), Juan Pujol al Este s/n (3470), Corrientes, Mercedes, Argentina; ANDERSON SARAVIA DE MELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO UBIOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO LORES, Laboratorio de Inmunomodulación y Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay; ALEJANDRA VICTORIA CAPOZZO, Instituto de Virología e Innovaciones Tecnológicas, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), INTA, Buenos Aires, Hurlingham, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; TERESA FREIRE, Laboratorio de Inmunomodulación y Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo. |
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Título : |
Fasciola hepatica infection modifies lgG1 specific immune response to foot-and-mouth disease virus induced by vaccination. |
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Fecha de publicación : |
2024 |
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Fuente / Imprenta : |
Vaccine. 2024, Volume 42, Issue 3, Pages 541-547. https://doi.org/10.1016/j.vaccine.2023.12.067 |
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ISSN : |
0264-410X |
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DOI : |
10.1016/j.vaccine.2023.12.067 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 19 August 2023; Received in revised form 15 December 2023; Accepted 20 December 2023; Available online 6 January 2024. -- Correspondence: Freire, T.; UdelaR, Facultad de Medicina, Departamento de Inmunobiología, Gral. Flores 2125, Montevideo, Uruguay; email:tfreire@fmed.edu.uy -- Funding: Financial supports were provided by Programa de Desarrollo de Ciencias Básicas ( PEDECIBA ), Comisión Sectorial de Investigación Científica and Agencia Nacional de Investigación e Innovación (SNI-ANII and FCE_1_2019_1_156295) to Teresa Freire. Funding by GFRA Research to Florencia Mansilla was also provided. M. Costa was funded by ANII and CAP. A. V. Capozzo is a researcher of CONICET , Argentina. -- Supplementary data to this article can be found online at https://doi.org/10.1016/j.vaccine.2023.12.067 |
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Contenido : |
ABSTRACT.- Fasciola hepatica, a worldwide distributed helminth, has a robust immunoregulatory effect in the host, increasing the susceptibility to secondary infections. Foot and mouth disease (FMD) is a highly contagious acute vesicular viral disease effectively controlled by vaccination in endemic regions. Despite the evidence of immunoregulatory effects, the impact of fasciolosis on the immune response induced by FMD vaccination in cattle has never been assessed. Our objective was to evaluate whether the infection by F. hepatica in cattle influences the long-term immunity elicited by the currently used commercial FMD-inactivated vaccines. Aberdeen Angus steers negative for F. hepatica were vaccinated twice against FMD virus (FMDV) during the first 6 months of age using a commercial oil vaccine formulated with A24/Cruzeiro and O1/Campos strains. When maternal antibodies against F. hepatica were weaned (18--20 months of age) animals were divided into groups of 12 and infected or mock-infected with 500 metacercariae/animal. Individual serum samples were collected at 0-, 28-, 59-, 87- and 157-days post-infection (dpi). Indirect ELISAs were used to detect A24/Cruzeiro specific bovine IgG and IgG subtypes. The total IgG antibody levels and avidity against FMDV did not show significant differences between all the groups. The commercial vaccine induced higher IgG2 than IgG1 titers in vaccinated animals. Anti-FMDV IgG1 levels significantly decreased in the infected group at 28 dpi. In addition, the avidity of IgG1 FMDV-specific antibodies at day 28 in the infected group was reduced compared to the control. These results show that F. hepatica infection modified anamnestic responses against FMDV, reducing serum IgG1 titers and avidity. To our knowledge, this is the first report of immune-regulation of F. hepatica altering the immune response of FMD vaccines, one of the most globally used animal vaccines. © 2024 Elsevier Ltd MenosABSTRACT.- Fasciola hepatica, a worldwide distributed helminth, has a robust immunoregulatory effect in the host, increasing the susceptibility to secondary infections. Foot and mouth disease (FMD) is a highly contagious acute vesicular viral disease effectively controlled by vaccination in endemic regions. Despite the evidence of immunoregulatory effects, the impact of fasciolosis on the immune response induced by FMD vaccination in cattle has never been assessed. Our objective was to evaluate whether the infection by F. hepatica in cattle influences the long-term immunity elicited by the currently used commercial FMD-inactivated vaccines. Aberdeen Angus steers negative for F. hepatica were vaccinated twice against FMD virus (FMDV) during the first 6 months of age using a commercial oil vaccine formulated with A24/Cruzeiro and O1/Campos strains. When maternal antibodies against F. hepatica were weaned (18--20 months of age) animals were divided into groups of 12 and infected or mock-infected with 500 metacercariae/animal. Individual serum samples were collected at 0-, 28-, 59-, 87- and 157-days post-infection (dpi). Indirect ELISAs were used to detect A24/Cruzeiro specific bovine IgG and IgG subtypes. The total IgG antibody levels and avidity against FMDV did not show significant differences between all the groups. The commercial vaccine induced higher IgG2 than IgG1 titers in vaccinated animals. Anti-FMDV IgG1 levels significantly decreased in the infected group at 28 dpi.... Presentar Todo |
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Palabras claves : |
Fasciolosis; FMD vaccine; IgG avidity; Immune modulation; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Vaccine efficacy. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 03751naa a2200313 a 4500
001 1064474
005 2024-02-26
008 2024 bl uuuu u00u1 u #d
022 $a0264-410X
024 7 $a10.1016/j.vaccine.2023.12.067$2DOI
100 1 $aCOSTA, M.
245 $aFasciola hepatica infection modifies lgG1 specific immune response to foot-and-mouth disease virus induced by vaccination.$h[electronic resource]
260 $c2024
500 $aArticle history: Received 19 August 2023; Received in revised form 15 December 2023; Accepted 20 December 2023; Available online 6 January 2024. -- Correspondence: Freire, T.; UdelaR, Facultad de Medicina, Departamento de Inmunobiología, Gral. Flores 2125, Montevideo, Uruguay; email:tfreire@fmed.edu.uy -- Funding: Financial supports were provided by Programa de Desarrollo de Ciencias Básicas ( PEDECIBA ), Comisión Sectorial de Investigación Científica and Agencia Nacional de Investigación e Innovación (SNI-ANII and FCE_1_2019_1_156295) to Teresa Freire. Funding by GFRA Research to Florencia Mansilla was also provided. M. Costa was funded by ANII and CAP. A. V. Capozzo is a researcher of CONICET , Argentina. -- Supplementary data to this article can be found online at https://doi.org/10.1016/j.vaccine.2023.12.067
520 $aABSTRACT.- Fasciola hepatica, a worldwide distributed helminth, has a robust immunoregulatory effect in the host, increasing the susceptibility to secondary infections. Foot and mouth disease (FMD) is a highly contagious acute vesicular viral disease effectively controlled by vaccination in endemic regions. Despite the evidence of immunoregulatory effects, the impact of fasciolosis on the immune response induced by FMD vaccination in cattle has never been assessed. Our objective was to evaluate whether the infection by F. hepatica in cattle influences the long-term immunity elicited by the currently used commercial FMD-inactivated vaccines. Aberdeen Angus steers negative for F. hepatica were vaccinated twice against FMD virus (FMDV) during the first 6 months of age using a commercial oil vaccine formulated with A24/Cruzeiro and O1/Campos strains. When maternal antibodies against F. hepatica were weaned (18--20 months of age) animals were divided into groups of 12 and infected or mock-infected with 500 metacercariae/animal. Individual serum samples were collected at 0-, 28-, 59-, 87- and 157-days post-infection (dpi). Indirect ELISAs were used to detect A24/Cruzeiro specific bovine IgG and IgG subtypes. The total IgG antibody levels and avidity against FMDV did not show significant differences between all the groups. The commercial vaccine induced higher IgG2 than IgG1 titers in vaccinated animals. Anti-FMDV IgG1 levels significantly decreased in the infected group at 28 dpi. In addition, the avidity of IgG1 FMDV-specific antibodies at day 28 in the infected group was reduced compared to the control. These results show that F. hepatica infection modified anamnestic responses against FMDV, reducing serum IgG1 titers and avidity. To our knowledge, this is the first report of immune-regulation of F. hepatica altering the immune response of FMD vaccines, one of the most globally used animal vaccines. © 2024 Elsevier Ltd
653 $aFasciolosis
653 $aFMD vaccine
653 $aIgG avidity
653 $aImmune modulation
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aVaccine efficacy
700 1 $aMANSILLA, F.
700 1 $aMANUEL SALA, J.
700 1 $aSARAVIA, A.
700 1 $aUBIOS, D.
700 1 $aLORES, P.
700 1 $aCAPOZZO, A.V.
700 1 $aFREIRE, T.
773 $tVaccine. 2024, Volume 42, Issue 3, Pages 541-547. https://doi.org/10.1016/j.vaccine.2023.12.067
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
27/01/2021 |
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Actualizado : |
24/02/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SALVO, M.; REY, F.; ARRUABARRENA, A.; GAMBETTA, G.; RODRIGO, M. J.; ZACARÍAS, L.; LADO, J. |
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Afiliación : |
MATÍAS SALVO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FLORENCIA REY, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Paterna, Valencia, Spain; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GIULIANA GAMBETTA, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; MARÍA J. RODRIGO, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Paterna, Valencia, Spain; LORENZO ZACARÍAS, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Paterna, Valencia, Spain; JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Transcriptional analysis of C-repeat binding factors in fruit of citrus species with differential sensitivity to chilling injury during postharvest storage. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
International Journal of Molecular Sciences, 2 January 2021, Volume 22, Issue 2, Article number 804, Pages 1-20. OPEN ACCESS. Doi: https://doi.org/10.3390/ijms22020804 |
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ISSN : |
1661-6596 |
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DOI : |
10.3390/ijms22020804 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 9 December 2020; Revised 5 January 2021; Accepted 12 January 2021; Published 15 January 2021.
This work was supported by a research grant from the National Agency of Research and Innovation (ANII-Uruguay) by the code FCE_3_2016_1_126714 and by a research grant RTI2018? 095131-B-I00 of the Ministry of Science and Innovation (Spanish Government). F. Rey is the recipient of a predoctoral scholarship (POS_EXT_2016_1_133720) from ANII (Uruguay).
Supplementary material.
This article belongs to the Special Issue Gene Transcriptional Regulation in Crops during Postharvest. |
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Contenido : |
ABSTRACT.
Citrus fruit are sensitive to chilling injury (CI) during cold storage, a peel disorder that causes economic losses. C-repeat binding factors (CBFs) are related to cold acclimation and tolerance in different plants. To explore the role of Citrus CBFs in fruit response to cold, an in silico study was performed, revealing three genes (CBF1, CBF2, and CBF3) whose expression in CI sensitive and tolerant cultivars was followed. Major changes occurred at the early stages of cold exposure (1?5 d). Interestingly, CBF1 was the most stimulated gene in the peel of CI-tolerant cultivars (Lisbon lemon, Star Ruby grapefruit, and Navelina orange), remaining unaltered in sensitive cultivars (Meyer lemon, Marsh grapefruit, and Salustiana orange). Results suggest a positive association of CBF1 expression with cold tolerance in Citrus cultivars (except for mandarins), whereas the expression of CBF2 or CBF3 genes did not reveal a clear relationship with the susceptibility to CI. Light avoidance during fruit growth reduced postharvest CI in most sensitive cultivars, associated with a rapid and transient enhance in the expression of the three CBFs. Results suggest that CBFs-dependent pathways mediate at least part of the cold tolerance responses in sensitive Citrus, indicating that CBF1 participates in the natural tolerance to CI. |
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Palabras claves : |
CBF; Chilling injury; DREB; Gene expression; PLATAFORMA AGROALIMENTOS. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
https://www.mdpi.com/1422-0067/22/2/804
https://www.mdpi.com/1422-0067/22/2/804/pdf
https://www.mdpi.com/1422-0067/22/2/804/s1
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Marc : |
LEADER 02929naa a2200301 a 4500
001 1061688
005 2022-02-24
008 2021 bl uuuu u00u1 u #d
022 $a1661-6596
024 7 $a10.3390/ijms22020804$2DOI
100 1 $aSALVO, M.
245 $aTranscriptional analysis of C-repeat binding factors in fruit of citrus species with differential sensitivity to chilling injury during postharvest storage.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 9 December 2020; Revised 5 January 2021; Accepted 12 January 2021; Published 15 January 2021. This work was supported by a research grant from the National Agency of Research and Innovation (ANII-Uruguay) by the code FCE_3_2016_1_126714 and by a research grant RTI2018? 095131-B-I00 of the Ministry of Science and Innovation (Spanish Government). F. Rey is the recipient of a predoctoral scholarship (POS_EXT_2016_1_133720) from ANII (Uruguay). Supplementary material. This article belongs to the Special Issue Gene Transcriptional Regulation in Crops during Postharvest.
520 $aABSTRACT. Citrus fruit are sensitive to chilling injury (CI) during cold storage, a peel disorder that causes economic losses. C-repeat binding factors (CBFs) are related to cold acclimation and tolerance in different plants. To explore the role of Citrus CBFs in fruit response to cold, an in silico study was performed, revealing three genes (CBF1, CBF2, and CBF3) whose expression in CI sensitive and tolerant cultivars was followed. Major changes occurred at the early stages of cold exposure (1?5 d). Interestingly, CBF1 was the most stimulated gene in the peel of CI-tolerant cultivars (Lisbon lemon, Star Ruby grapefruit, and Navelina orange), remaining unaltered in sensitive cultivars (Meyer lemon, Marsh grapefruit, and Salustiana orange). Results suggest a positive association of CBF1 expression with cold tolerance in Citrus cultivars (except for mandarins), whereas the expression of CBF2 or CBF3 genes did not reveal a clear relationship with the susceptibility to CI. Light avoidance during fruit growth reduced postharvest CI in most sensitive cultivars, associated with a rapid and transient enhance in the expression of the three CBFs. Results suggest that CBFs-dependent pathways mediate at least part of the cold tolerance responses in sensitive Citrus, indicating that CBF1 participates in the natural tolerance to CI.
650 $aCITRUS
653 $aCBF
653 $aChilling injury
653 $aDREB
653 $aGene expression
653 $aPLATAFORMA AGROALIMENTOS
700 1 $aREY, F.
700 1 $aARRUABARRENA, A.
700 1 $aGAMBETTA, G.
700 1 $aRODRIGO, M. J.
700 1 $aZACARÍAS, L.
700 1 $aLADO, J.
773 $tInternational Journal of Molecular Sciences, 2 January 2021, Volume 22, Issue 2, Article number 804, Pages 1-20. OPEN ACCESS. Doi: https://doi.org/10.3390/ijms22020804
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